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| Item Type: | Article |
|---|---|
| Title: | Rat cytomegalovirus efficiently replicates in dendritic cells and induces changes in their transcriptional profile |
| Creators Name: | Madela-Mönchinger, J.C., Wolf, S.A., Wyler, E., Bauer, A., Mischke, M., Möller, L., Juranić Lisnić, V., Landthaler, M., Malyshkina, A. and Voigt, S. |
| Abstract: | Dendritic cells (DC) play a crucial role in generating and maintaining antiviral immunity. While DC are implicated in the antiviral defense by inducing T cell responses, they can also become infected by Cytomegalovirus (CMV). CMV is not only highly species-specific but also specialized in evading immune protection, and this specialization is in part due to characteristic genes encoded by a given virus. Here, we investigated whether rat CMV can infect XCR1(+) DC and if infection of DC alters expression of cell surface markers and migration behavior. We demonstrate that wild-type RCMV and a mutant virus lacking the γ-chemokine ligand xcl1 (Δvxcl1 RCMV) infect splenic rat DC ex vivo and identify viral assembly compartments. Replication-competent RCMV reduced XCR1 and MHCII surface expression. Further, gene expression of infected DC was analyzed by bulk RNA-sequencing (RNA-Seq). RCMV infection reverted a state of DC activation that was induced by DC cultivation. On the functional level, we observed impaired chemotactic activity of infected XCR1(+) DC compared to mock-treated cells. We therefore speculate that as a result of RCMV infection, DC exhibit diminished XCR1 expression and are thereby blocked from the lymphocyte crosstalk. |
| Keywords: | Cytomegalovirus, Dendritic Cells, Transcriptome, Chemokines, XCL1, XCR1, Animals, Rats |
| Source: | Frontiers in Immunology |
| ISSN: | 1664-3224 |
| Publisher: | Frontiers Media SA |
| Volume: | 14 |
| Page Range: | 1192057 |
| Date: | 23 November 2023 |
| Official Publication: | https://doi.org/10.3389/fimmu.2023.1192057 |
| PubMed: | View item in PubMed |
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