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| Item Type: | Article |
|---|---|
| Title: | Generation of an NCS1 gene knockout human induced pluripotent stem cell line using CRISPR/Cas9 |
| Creators Name: | Maierhof, S.K., Schinke, C., Cernoch, J., Hew, L., Bruske, L.P., Fernandez Vallone, V., Fischer, K., Stachelscheid, H., Huehnchen, P., Endres, M., Diecke, S., Telugu, N.S. and Boehmerle, W. |
| Abstract: | NCS1 (Neuronal calcium sensor protein 1) encodes a highly conserved calcium binding protein abundantly expressed in neurons. It modulates intracellular calcium homeostasis, calcium-dependent signaling pathways as well as neuronal transmission and plasticity. Here, we generated a NCS1 knockout human induced pluripotent stem cell (hiPSC) line using CRISPR-Cas9 genome editing. It shows regular expression of pluripotent markers, normal iPSC morphology and karyotype as well as no detectable off-target effects on top 6 potentially affected genes. This newly generated cell line constitutes a valuable tool for studying the role of NCS1 in the pathophysiology of various neuropsychiatric disorders and non-neurological disease. |
| Keywords: | Calcium, CRISPR-Cas Systems, Gene Editing, Gene Knockout Techniques, Induced Pluripotent Stem Cells |
| Source: | Stem Cell Research |
| ISSN: | 1873-5061 |
| Publisher: | Elsevier |
| Volume: | 73 |
| Page Range: | 103253 |
| Date: | December 2023 |
| Official Publication: | https://doi.org/10.1016/j.scr.2023.10325 |
| PubMed: | View item in PubMed |
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