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| Item Type: | Article |
|---|---|
| Title: | Mislocalization of pathogenic RBM20 variants in dilated cardiomyopathy is caused by loss-of-interaction with Transportin-3 |
| Creators Name: | Kornienko, J., Rodríguez-Martínez, M., Fenzl, K., Hinze, F., Schraivogel, D., Grosch, M., Tunaj, B., Lindenhofer, D., Schraft, L., Kueblbeck, M., Smith, E., Mao, C., Brown, E., Owens, A., Saguner, A.M., Meder, B., Parikh, V., Gotthardt, M. and Steinmetz, L.M. |
| Abstract: | Severe forms of dilated cardiomyopathy (DCM) are associated with point mutations in the alternative splicing regulator RBM20 that are frequently located in the arginine/serine-rich domain (RS-domain). Such mutations can cause defective splicing and cytoplasmic mislocalization, which leads to the formation of detrimental cytoplasmic granules. Successful development of personalized therapies requires identifying the direct mechanisms of pathogenic RBM20 variants. Here, we decipher the molecular mechanism of RBM20 mislocalization and its specific role in DCM pathogenesis. We demonstrate that mislocalized RBM20 RS-domain variants retain their splice regulatory activity, which reveals that aberrant cellular localization is the main driver of their pathological phenotype. A genome-wide CRISPR knockout screen combined with image-enabled cell sorting identified Transportin-3 (TNPO3) as the main nuclear importer of RBM20. We show that the direct RBM20-TNPO3 interaction involves the RS-domain, and is disrupted by pathogenic variants. Relocalization of pathogenic RBM20 variants to the nucleus restores alternative splicing and dissolves cytoplasmic granules in cell culture and animal models. These findings provide proof-of-principle for developing therapeutic strategies to restore RBM20's nuclear localization in RBM20-DCM patients. |
| Keywords: | Alternative Splicing, Dilated Cardiomyopathy, Karyopherins, Mutation, RNA Splicing, Animals |
| Source: | Nature Communications |
| ISSN: | 2041-1723 |
| Publisher: | Nature Publishing Group |
| Volume: | 14 |
| Number: | 1 |
| Page Range: | 4312 |
| Date: | 18 July 2023 |
| Official Publication: | https://doi.org/10.1038/s41467-023-39965-6 |
| PubMed: | View item in PubMed |
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