![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
Preview |
PDF (Original Article)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
6MB |
![[thumbnail of Supporting Information]](https://edoc.mdc-berlin.de/style/images/fileicons/other.png) |
Other (Supporting Information)
5MB |
| Item Type: | Article |
|---|---|
| Title: | SLAM-Drop-seq reveals mRNA kinetic rates throughout the cell cycle |
| Creators Name: | Liu, H., Arsiè, R., Schwabe, D., Schilling, M., Minia, I., Alles, J., Boltengagen, A., Kocks, C., Falcke, M., Friedman, N., Landthaler, M. and Rajewsky, N. |
| Abstract: | RNA abundance is tightly regulated in eukaryotic cells by modulating the kinetic rates of RNA production, processing, and degradation. To date, little is known about time-dependent kinetic rates during dynamic processes. Here, we present SLAM-Drop-seq, a method that combines RNA metabolic labeling and alkylation of modified nucleotides in methanol-fixed cells with droplet-based sequencing to detect newly synthesized and preexisting mRNAs in single cells. As a first application, we sequenced 7280 HEK293 cells and calculated gene-specific kinetic rates during the cell cycle using the novel package Eskrate. Of the 377 robust-cycling genes that we identified, only a minor fraction is regulated solely by either dynamic transcription or degradation (6 and 4%, respectively). By contrast, the vast majority (89%) exhibit dynamically regulated transcription and degradation rates during the cell cycle. Our study thus shows that temporally regulated mRNA degradation is fundamental for the correct expression of a majority of cycling genes. SLAM-Drop-seq, combined with Eskrate, is a powerful approach to understanding the underlying mRNA kinetics of single-cell gene expression dynamics in continuous biological processes. |
| Keywords: | Cell Cycle, mRNA Kinetics, Single Cells, Temporal Regulation, Transcription and Degradation |
| Source: | Molecular Systems Biology |
| ISSN: | 1744-4292 |
| Publisher: | Wiley |
| Volume: | 19 |
| Number: | 10 |
| Page Range: | e11427 |
| Date: | 12 October 2023 |
| Official Publication: | https://doi.org/10.15252/msb.202211427 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
