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| Item Type: | Article |
|---|---|
| Title: | Calcium-permeable channelrhodopsins for the photocontrol of calcium signalling |
| Creators: |
Fernandez Lahore, R.G.  ORCID: https://orcid.org/0000-0002-8559-2767, Pampaloni, N.P. ORCID: https://orcid.org/0000-0002-4066-0295, Schiewer, E. ORCID: https://orcid.org/0000-0001-7913-5597, Heim, M.M. ORCID: https://orcid.org/0000-0002-8079-4204, Tillert, L. ORCID: https://orcid.org/0000-0001-7497-018X, Vierock, J., Oppermann, J. ORCID: https://orcid.org/0000-0002-3442-1458, Walther, J. ORCID: https://orcid.org/0000-0001-6254-9128, Schmitz, D. ORCID: https://orcid.org/0000-0003-2741-5241, Owald, D. ORCID: https://orcid.org/0000-0001-7747-7884, Plested, A.J.R. ORCID: https://orcid.org/0000-0001-6062-0832, Rost, B.R. ORCID: https://orcid.org/0000-0003-1906-0081 and Hegemann, P. ORCID: https://orcid.org/0000-0003-3589-6452
|
| Abstract: | Channelrhodopsins are light-gated ion channels used to control excitability of designated cells in large networks with high spatiotemporal resolution. While ChRs selective for H(+), Na(+), K(+) and anions have been discovered or engineered, Ca(+)-selective ChRs have not been reported to date. Here, we analyse ChRs and mutant derivatives with regard to their Ca(+) permeability and improve their Ca(+) affinity by targeted mutagenesis at the central selectivity filter. The engineered channels, termed CapChR1 and CapChR2 for calcium-permeable channelrhodopsins, exhibit reduced sodium and proton conductance in connection with strongly improved Ca(+) permeation at negative voltage and low extracellular Ca(+) concentrations. In cultured cells and neurons, CapChR2 reliably increases intracellular Ca(+) concentrations. Moreover, CapChR2 can robustly trigger Ca(+) signalling in hippocampal neurons. When expressed together with genetically encoded Ca(+) indicators in Drosophila melanogaster mushroom body output neurons, CapChRs mediate light-evoked Ca(+) entry in brain explants. |
| Keywords: | Cellular Neuroscience, Optogenetics, Permeation and Transport, Animals, Drosophila Melanogaster |
| Source: | Nature Communications |
| ISSN: | 2041-1723 |
| Publisher: | Nature Publishing Group |
| Volume: | 13 |
| Number: | 1 |
| Page Range: | 7844 |
| Date: | 21 December 2022 |
| Additional Information: | Erratum in : Nat Commun 15(1):3958. |
| Official Publication: | https://doi.org/10.1038/s41467-022-35373-4 |
| PubMed: | View item in PubMed |
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