Pillars article: independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting

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Item Type:	Article
Title:	Pillars article: independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting
Creators Name:	Gu, H., Zou, Y.R. and Rajewsky, K.
Abstract:	We have employed a method based on the Cre-loxP recombination system of bacteriophage P1 to generate a mouse strain in which the JH segments and the intron enhancer in the IgH locus are deleted. By analysis of immunoglobulin isotype switch recombination in heterozygous mutant B cells activated by lipopolysaccharide plus interleukin-4, we show that, on the mutant chromosome, switch recombination at the μ gene switch region is strongly suppressed, whereas the switch region of the γ1 gene is efficiently rearranged. These data demonstrate an independent control of switch recombination at individual switch regions and suggest that, in the process of switch recombination, the alignment of the recombining strands occurs independently of and probably after the introduction of double-strand breaks into the switch regions involved.
Keywords:	Base Sequence, Cultured Tumor Cells, Genetic Recombination, Heavy Chain B-Lymphocyte Gene Rearrangement, Immunoglobulin Genes, Inbred C57BL Mice, Molecular Sequence Data, Mutant Strains Mice, Switch Genes, Animals, Mice
Source:	Cell
ISSN:	0092-8674
Publisher:	Cell Press
Volume:	73
Number:	6
Page Range:	1155-1164
Date:	18 June 1993
Official Publication:	https://doi.org/10.1016/0092-8674(93)90644-6
PubMed:	View item in PubMed

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