Transposon mapping using flanking sequence exponential anchored (FLEA) PCR

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Item Type:	Preprint
Title:	Transposon mapping using flanking sequence exponential anchored (FLEA) PCR
Creators Name:	Henssen, A., Carson, J. and Kentsis, A.
Abstract:	This protocol describes a modied version of anking sequence exponential anchored \(FLEA) PCR. The original FLEA-PCR method was developed to amplify integration sites of retroviruses or retrotransposons. Such transposons contain two long terminal repeats \(LTR), which have the same orientation. In contrast, DNA transposons have inverted terminal repeats \(ITR), which are inverted in their orientation. Here, we adapt FLEA-PCR for mapping genomic DNA transposition, suitable for the analysis of DNA transposition reporters and genomes engineered through the use of DNA transposition.
Keywords:	Transposition, Genomic Integration, PCR, Flanking Sequence PCR
Source:	Protocol Exchange
ISSN:	2043-0116
Publisher:	Research Square
Article Number:	2015.071
Date:	22 July 2015
Official Publication:	https://doi.org/10.1038/protex.2015.071

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