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Intranasal SARS-CoV-2 spike-based immunisation adjuvanted with polyethyleneimine elicits mucosal and systemic humoral responses in mice

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Item Type:Article
Title:Intranasal SARS-CoV-2 spike-based immunisation adjuvanted with polyethyleneimine elicits mucosal and systemic humoral responses in mice
Creators Name:Deimel, L.P., Liu, X., Gilbert-Jaramillo, J., Liu, S., James, W.S. and Sattentau, Q.J.
Abstract:The SARS-CoV-2 pandemic continues despite the presence of effective vaccines, and novel vaccine approaches may help to reduce viral spread and associated COVID-19 disease. Current vaccine administration modalities are based on systemic needle-administered immunisation which may be suboptimal for mucosal pathogens. Here we demonstrate in a mouse model that small-volume intranasal administration of purified spike (S) protein in the adjuvant polyethylenemine (PEI) elicits robust antibody responses with modest systemic neutralisation activity. Further, we test a heterologous intranasal immunisation regimen, priming with S and boosting with RBD-Fc. Our data identify small volume PEI adjuvantation as a novel platform with potential for protective mucosal vaccine development.
Keywords:SARS-CoV-2, COVID-19, Spike, RBD, Mucosal Adjuvant, Adaptive Immunity, Vaccine, Animals, Mice
Source:Journal of Immunological Methods
ISSN:0022-1759
Publisher:Elsevier
Volume:511
Page Range:113380
Date:December 2022
Official Publication:https://doi.org/10.1016/j.jim.2022.113380
PubMed:View item in PubMed

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