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| Item Type: | Article |
|---|---|
| Title: | Intranasal SARS-CoV-2 spike-based immunisation adjuvanted with polyethyleneimine elicits mucosal and systemic humoral responses in mice |
| Creators Name: | Deimel, L.P., Liu, X., Gilbert-Jaramillo, J., Liu, S., James, W.S. and Sattentau, Q.J. |
| Abstract: | The SARS-CoV-2 pandemic continues despite the presence of effective vaccines, and novel vaccine approaches may help to reduce viral spread and associated COVID-19 disease. Current vaccine administration modalities are based on systemic needle-administered immunisation which may be suboptimal for mucosal pathogens. Here we demonstrate in a mouse model that small-volume intranasal administration of purified spike (S) protein in the adjuvant polyethylenemine (PEI) elicits robust antibody responses with modest systemic neutralisation activity. Further, we test a heterologous intranasal immunisation regimen, priming with S and boosting with RBD-Fc. Our data identify small volume PEI adjuvantation as a novel platform with potential for protective mucosal vaccine development. |
| Keywords: | SARS-CoV-2, COVID-19, Spike, RBD, Mucosal Adjuvant, Adaptive Immunity, Vaccine, Animals, Mice |
| Source: | Journal of Immunological Methods |
| ISSN: | 0022-1759 |
| Publisher: | Elsevier |
| Volume: | 511 |
| Page Range: | 113380 |
| Date: | December 2022 |
| Official Publication: | https://doi.org/10.1016/j.jim.2022.113380 |
| PubMed: | View item in PubMed |
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