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| Item Type: | Preprint |
|---|---|
| Title: | The selection arena in early human blastocysts resolves the pluripotent inner cell mass |
| Creators Name: | Singh, M., Widmann, T.J., Bansal, V., Cortes, J.L., Schumann, G.G., Wunderlich, S., Martin, U., Garcia-Canadas, M., Garcia-Perez, J.L., Hurst, L.D. and Izsvák, Z. |
| Abstract: | Is the human early embryo unique in lacking an inner cell-mass (ICM) and having parallel development? We reanalyse single-cell transcriptomic data and stain human embryos in situ to reveal both classical step-wise development and a transcriptomically homologous ICM. This apparent classicism obscures phylogenetic singularity: unlike mice, human epiblast has self-renewal hallmarks and we have abundant blastocyst non-committed cells (NCCs), part of an apoptosis-mediated purging process. The transcriptomes of the pluripotent cells are fast evolving, in large part owing to endogenous retrovirus H (ERVH) activity, rendering all primate embryos unique. Each species is characterised by the ERVHs that are active and the neighbour genes whose expression are modulated. ERVH is associated with recent major gene expression gain and loss events of pluripotencyassociated genes. Not least through lack of HERVH expression, the current portfolio of naïve cultures, putative in vitro mimics of pluripotent cells, are both developmentally and phylogenetically “confused”. |
| Source: | bioRxiv |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Article Number: | 318329v3 |
| Date: | 4 February 2019 |
| Official Publication: | https://doi.org/10.1101/318329 |
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