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Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvae

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Item Type:Article
Title:Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvae
Creators Name:Corradi, L., Zaupa, M., Sawamiphak, S. and Filosa, A.
Abstract:The larval zebrafish has emerged as a very useful model organism to study the neuronal circuits controlling neuroendocrine and behavioral responses to stress. This protocol describes how to expose zebrafish larvae to hyperosmotic stress and test whether candidate populations of neurons are activated or inhibited by the stressor using a relatively rapid immunofluorescence staining approach. This approach takes advantage of the phosphorylation of the extracellular signal-regulated kinase (ERK) upon neuronal activation. For complete details on the use and execution of this protocol, please refer to Corradi et al. (2022).
Keywords:Microscopy, Model Organisms, Neuroscience Behavior, Animals, Zebrafish
Source:STAR Protocols
ISSN:2666-1667
Publisher:Cell Press / Elsevier
Volume:3
Number:4
Page Range:101731
Date:16 December 2022
Official Publication:https://doi.org/10.1016/j.xpro.2022.101731
PubMed:View item in PubMed

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