![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
Preview |
PDF (Original Article)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
6MB |
| Item Type: | Article |
|---|---|
| Title: | Repression of AP-1-stimulated transcription by c-Ets-1 |
| Creators Name: | Goldberg, Y., Treier, M., Ghysdael, J. and Bohmann, D. |
| Abstract: | The transcriptional activities of c-Ets-1 and v-Ets and their functional interaction with the AP-1 factor c-Jun were investigated. Several recombinant Ets proteins were produced and purified either from bacteria or from insect cells. Plasmid DNAs that contained the polyoma virus enhancer Ets/AP-1 element were used as templates for in vitro transcription assays in the presence of HeLa nuclear extract and various combinations of the Jun and Ets proteins. Under these conditions full-length c-Ets-1 on its own does not markedly influence transcription but abolishes the strong transcriptional stimulation normally elicited by Jun. This repression depends on the Ets-binding site and on specific features of c-Ets-1 structure, as both v-Ets and a natural splicing variant c-Ets-1 (delta VII) fail to inhibit Jun activity. These findings suggest that c-Ets may act both as a transcriptional repressor or activator depending on promoter context and splicing pattern. |
| Keywords: | Alternative Splicing, Amino Acid Sequence, Base Sequence, Binding Sites, DNA, Escherichia Coli, Genetic Transcription, HeLa Cells, Molecular Cloning, Molecular Sequence Data, Oligodeoxyribonucleotides, Oncogenic Retroviridae Proteins, Polymerase Chain Reaction, Polyomavirus, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins C-Ets, Proto-Oncogene Proteins C-Jun, Recombinant Proteins, TATA Box, Tetradecanoylphorbol Acetate, Transcription Factors, Transfection |
| Source: | Journal of Biological Chemistry |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Volume: | 269 |
| Number: | 24 |
| Page Range: | 16566-16573 |
| Date: | 17 June 1994 |
| Official Publication: | https://doi.org/10.1016/S0021-9258(19)89426-7 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
