Purification of recombinant Mycobacterium leprae 65-kilodalton antigen and murine antibody responses to intravenously administered recombinant protein

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Item Type:	Article
Title:	Purification of recombinant Mycobacterium leprae 65-kilodalton antigen and murine antibody responses to intravenously administered recombinant protein
Creators Name:	Char, S., Colston, M.J., Mitchison, N.A. and Simon, K.
Abstract:	Recombinant Mycobacterium leprae 65-kilodalton antigen has been purified by a combination of differential solubility, ion-exchange and hydrophobic interaction chromatography, and a method for the quantification of the antigen in large scale protein preparations developed. By injecting large amounts of the recombinant antigen intravenously into mice, a limited degree of specific suppression of antibody-response has been induced.
Keywords:	Bacterial Antibodies, Bacterial Antigens, Ion Exchange Chromatography, Densitometry, Enzyme-Linked Immunosorbent Assay, Immunoblotting, Inbred BALB C Mice, Intravenous Injections, Mycobacterium leprae, Recombinant Proteins, Solubility, Animals, Mice
Source:	Tropical Medicine and Parasitology
ISSN:	0177-2392
Publisher:	Trop Med Parasitol
Volume:	41
Number:	3
Page Range:	336-8
Date:	September 1990
Official Publication:	https://doi.org/
PubMed:	View item in PubMed

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