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| Item Type: | Article |
|---|---|
| Title: | Insights into pancreatic β cell energy metabolism using rodent β cell models |
| Creators Name: | Morten, K.J., Potter, M., Badder, L., Sivathondan, P., Dragovic, R., Neumann, A., Gavin, J., Shrestha, R., Reilly, S., Phadwal, K., Lodge, T.A., Borzychowski, A., Cookson, S., Mitchell, C., Morovat, A., Simon, A.K., Uusimaa, J., Hynes, J. and Poulton, J. |
| Abstract: | Mitochondrial diabetes is primarily caused by β-cell failure, a cell type whose unique properties are important in pathogenesis. By reducing glucose, we induced energetic stress in two rodent β-cell models to assess effects on cellular function. Culturing rat insulin-secreting INS-1 cells in low glucose conditions caused a rapid reduction in whole cell respiration, associated with elevated mitochondrial reactive oxygen species production, and an altered glucose-stimulated insulin secretion profile. Prolonged exposure to reduced glucose directly impaired mitochondrial function and reduced autophagy. Insulinoma cell lines have a very different bioenergetic profile to many other cell lines and provide a useful model of mechanisms affecting β-cell mitochondrial function. |
| Keywords: | Beta-Cell, Oxidative Phosphorylation, Reactive Oxygen Species, Superoxide, Mitochondria, Insulin Secretion |
| Source: | Wellcome Open Research |
| ISSN: | 2398-502X |
| Publisher: | Wellcome |
| Volume: | 2 |
| Page Range: | 14 |
| Date: | 25 September 2019 |
| Official Publication: | https://doi.org/10.12688/wellcomeopenres.10535.1 |
| PubMed: | View item in PubMed |
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