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| Item Type: | Article |
|---|---|
| Title: | Upregulation of SPS100 gene expression by an antisense RNA via a switch of mRNA isoforms with different stabilities |
| Creators Name: | Bunina, D., Štefl, M., Huber, F., Khmelinskii, A., Meurer, M., Barry, J.D., Kats, I., Kirrmaier, D., Huber, W. and Knop, M. |
| Abstract: | Pervasive transcription of genomes generates multiple classes of non-coding RNAs. One of these classes are stable long non-coding RNAs which overlap coding genes in antisense direction (asRNAs). The function of such asRNAs is not fully understood but several cases of antisense-dependent gene expression regulation affecting the overlapping genes have been demonstrated. Using high-throughput yeast genetics and a limited set of four growth conditions we previously reported a regulatory function for ∼25% of asRNAs, most of which repress the expression of the sense gene. To further explore the roles of asRNAs we tested more conditions and identified 15 conditionally antisense-regulated genes, 6 of which exhibited antisense-dependent enhancement of gene expression. We focused on the sporulation-specific gene SPS100, which becomes upregulated upon entry into starvation or sporulation as a function of the antisense transcript SUT169. We demonstrate that the antisense effect is mediated by its 3' intergenic region (3'-IGR) and that this regulation can be transferred to other genes. Genetic analysis revealed that SUT169 functions by changing the relative expression of SPS100 mRNA isoforms from a short and unstable transcript to a long and stable species. These results suggest a novel mechanism of antisense-dependent gene regulation via mRNA isoform switching. |
| Keywords: | Antisense RNA, Fluorescence Microscopy, Fungal Gene Expression Regulation, Fungal Spores, Immunoblotting, RNA Isoforms, RNA Stability, Reverse Transcriptase Polymerase Chain Reaction, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Time-Lapse Imaging, Up-Regulation |
| Source: | Nucleic Acids Research |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Volume: | 45 |
| Number: | 19 |
| Page Range: | 11144-11158 |
| Date: | 2 November 2017 |
| Official Publication: | https://doi.org/10.1093/nar/gkx737 |
| PubMed: | View item in PubMed |
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