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| Item Type: | Article |
|---|---|
| Title: | Analyses of murine lymph node endothelial cell subsets using single-cell RNA sequencing and spectral flow cytometry |
| Creators Name: | Menzel, L., Zschummel, M. and Rehm, A. |
| Abstract: | Blood endothelial cells (BECs) in lymph nodes are distinct stromal cells with a transcriptional profile allowing fast and specific adaptation to the functional requirements. Here, we describe a step-by-step protocol for the enzymatic digestion of lymph nodes, the enrichment of stromal cells, the sorting of BECs, and the processing of BEC-related data for modern analysis approaches as spectral flow cytometry and single-cell RNA sequencing (scRNA-seq). For complete details on the use and execution of this protocol, please refer to Menzel et al. (2021). |
| Keywords: | Bioinformatics, Cell Isolation, Single Cell, Flow Cytometry/Mass Cytometry, Cancer, Genomics, RNAseq, Immunology, Animals, Mice |
| Source: | STAR Protocols |
| ISSN: | 2666-1667 |
| Publisher: | Elsevier |
| Volume: | 3 |
| Number: | 2 |
| Page Range: | 101267 |
| Date: | 17 June 2022 |
| Official Publication: | https://doi.org/10.1016/j.xpro.2022.101267 |
| PubMed: | View item in PubMed |
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