Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

A single-cell Arabidopsis root atlas reveals developmental trajectories in wild-type and cell identity mutants

[thumbnail of Original Article]
Preview
PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
8MB
[thumbnail of Supplemental Information] Other (Supplemental Information)
79MB

Item Type:Article
Title:A single-cell Arabidopsis root atlas reveals developmental trajectories in wild-type and cell identity mutants
Creators Name:Shahan, R., Hsu, C.W., Nolan, T.M., Cole, B.J., Taylor, I.W., Greenstreet, L., Zhang, S., Afanassiev, A., Vlot, A.H.C., Schiebinger, G., Benfey, P.N. and Ohler, U.
Abstract:In all multicellular organisms, transcriptional networks orchestrate organ development. The Arabidopsis root, with its simple structure and indeterminate growth, is an ideal model for investigating the spatiotemporal transcriptional signatures underlying developmental trajectories. To map gene expression dynamics across root cell types and developmental time, we built a comprehensive, organ-scale atlas at single-cell resolution. In addition to estimating developmental progressions in pseudotime, we employed the mathematical concept of optimal transport to infer developmental trajectories and identify their underlying regulators. To demonstrate the utility of the atlas to interpret new datasets, we profiled mutants for two key transcriptional regulators at single-cell resolution, shortroot and scarecrow. We report transcriptomic and in vivo evidence for tissue trans-differentiation underlying a mixed cell identity phenotype in scarecrow. Our results support the atlas as a rich community resource for unraveling the transcriptional programs that specify and maintain cell identity to regulate spatiotemporal organ development.
Keywords:Transcriptomics, Arabidopsis, Root, scRNA-seq, Development, Cell Fate, SHORTROOT, SCARECROW
Source:Developmental Cell
ISSN:1534-5807
Publisher:Cell Press
Volume:57
Number:4
Page Range:543-560
Date:28 February 2022
Official Publication:https://doi.org/10.1016/j.devcel.2022.01.008
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library