![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
| Item Type: | Article |
|---|---|
| Title: | Reciprocal changes in the postnatal expression of the sarcolemmal Na+-Ca2+-exchanger and SERCA2 in rat heart |
| Creators Name: | Vetter, R., Studer, R., Reinecke, H., Kolar, F., Ostadalova, I. and Drexler, H. |
| Abstract: | The aim of this study was to examine the relationship between sarcolemmal Na(+)-Ca2+ exchangers and sarcoplasmic reticulum (SR) Ca(2+) -ATPase (SERCA2) expression and the developmental differences in cardiac Ca2+ handling. Postnatal steady-state mRNA and protein levels were analysed in rat ventricular myocardium by Northern and immunoblot analysis, respectively. This was compared to Na+ gradient-induced and SR oxalate-supported Ca2 transport in isolated membranes. Na(+)-Ca2+ exchanger mRNA declined by 75% between day 1 and 30, whereas SR Ca2+ ATPase mRNA levels increased by 97% during this period. The Na(+)-Ca2+ exchanger mRNA/Ca(2+)-ATPase mRNA ratio was found to be inversely related to post-natal age. The changes in mRNA levels were associated with corresponding developmental differences in the Ca2+ transport activities of the respective membrane proteins. In crude membranes, the Na(+)-dependent Ca2+ transport activity (at 75 microM Ca2+) declined gradually (P < 0.01; mean +/- S.E.) from 17.7 +/- 2.4 nmoles Ca2+/g wet tissue/2s at day 1-3 (n = 5) to a value of 4.2 +/- 1.1 at day 40 (n =4). Conversely, SR Ca2+ uptake increased (P < 0.01) 2.6-fold during this period. The inversely related changes in the post-natal expression and function of the Na(+)-Ca2+ exchanger and SR Ca(2+)-ATPase suggest a coordinated control at the pretranslational level of the cellular Ca2+ transport processes mediated by the two membrane proteins. |
| Keywords: | Growth, Sarcoplasmic Reticulum, Sarcolemma, Calcium ATPase, Sodium-Calcium Exchange, Development, mRNA, Postextrasystolic Potentiation, Gene Expression, Animals, Rats |
| Source: | Journal of Molecular and Cellular Cardiology |
| ISSN: | 0022-2828 |
| Publisher: | Elsevier |
| Volume: | 27 |
| Number: | 8 |
| Page Range: | 1689-1701 |
| Date: | 1 August 1995 |
| Official Publication: | https://doi.org/10.1016/S0022-2828(95)90788-2 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
