Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis

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Item Type:	Article
Title:	Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis
Creators Name:	Moreno-Layseca, P., Jäntti, N.Z., Godbole, R., Sommer, C., Jacquemet, G., Al-Akhrass, H., Conway, J.R.W., Kronqvist, P., Kallionpää, R.E., Oliveira-Ferrer, L., Cervero, P., Linder, S., Aepfelbacher, M., Zauber, H., Rae, J., Parton, R.G., Disanza, A., Scita, G., Mayor, S., Selbach, M., Veltel, S. and Ivaska, J.
Abstract:	Spatially controlled, cargo-specific endocytosis is essential for development, tissue homeostasis and cancer invasion. Unlike cargo-specific clathrin-mediated endocytosis, the clathrin- and dynamin-independent endocytic pathway (CLIC-GEEC, CG pathway) is considered a bulk internalization route for the fluid phase, glycosylated membrane proteins and lipids. While the core molecular players of CG-endocytosis have been recently defined, evidence of cargo-specific adaptors or selective uptake of proteins for the pathway are lacking. Here we identify the actin-binding protein Swiprosin-1 (Swip1, EFHD2) as a cargo-specific adaptor for CG-endocytosis. Swip1 couples active Rab21-associated integrins with key components of the CG-endocytic machinery - Arf1, IRSp53 and actin - and is critical for integrin endocytosis. Through this function, Swip1 supports integrin-dependent cancer-cell migration and invasion, and is a negative prognostic marker in breast cancer. Our results demonstrate a previously unknown cargo selectivity for the CG pathway and a role for specific adaptors in recruitment into this endocytic route.
Keywords:	Actins, Biological Transport, Breast Neoplasms, Cell Movement, Clathrin, Dynamins, Endocytosis, Integrin beta1, Intracellular Signaling Peptides and Proteins, rab GTP-Binding Proteins
Source:	Nature Cell Biology
ISSN:	1465-7392
Publisher:	Nature Publishing Group
Volume:	23
Number:	10
Page Range:	1073-1084
Date:	October 2021
Additional Information:	"Erratum in: Nat Cell Biol https://doi.org/10.1038/s41556-021-00767-x, published online 6 October 2021."
Official Publication:	https://doi.org/10.1038/s41556-021-00767-x
PubMed:	View item in PubMed

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