Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

KAHRP dynamically relocalizes to remodeled actin junctions and associates with knob spirals in Plasmodium falciparum-infected erythrocytes

[thumbnail of Original Article]
Preview
PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
3MB
[thumbnail of Supporting Information]
Preview
PDF (Supporting Information) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
13MB

Item Type:Article
Title:KAHRP dynamically relocalizes to remodeled actin junctions and associates with knob spirals in Plasmodium falciparum-infected erythrocytes
Creators Name:Sanchez, C.P., Patra, P., Chang, S.Y.S., Karathanasis, C., Hanebutte, L., Kilian, N., Cyrklaff, M., Heilemann, M., Schwarz, U.S., Kudryashev, M. and Lanzer, M.
Abstract:The knob-associated histidine-rich protein (KAHRP) plays a pivotal role in the pathophysiology of Plasmodium falciparum malaria by forming membrane protrusions in infected erythrocytes, which anchor parasite-encoded adhesins to the membrane skeleton. The resulting sequestration of parasitized erythrocytes in the microvasculature leads to severe disease. Despite KAHRP being an important virulence factor, its physical location within the membrane skeleton is still debated, as is its function in knob formation. Here, we show by super-resolution microscopy that KAHRP initially associates with various skeletal components, including ankyrin bridges, but eventually colocalizes with remnant actin junctions. We further present a 35 Å map of the spiral scaffold underlying knobs and show that a KAHRP-targeting nanoprobe binds close to the spiral scaffold. Single-molecule localization microscopy detected ~60 KAHRP molecules/knob. We propose a dynamic model of KAHRP organization and a function of KAHRP in attaching other factors to the spiral scaffold.
Keywords:Actin, Ankyrin, Erythrocyte, Malaria, Microscopy, Virulence Factor
Source:Molecular Microbiology
ISSN:0950-382X
Publisher:Wiley
Volume:117
Number:2
Page Range:274-292
Date:February 2022
Official Publication:https://doi.org/10.1111/mmi.14811
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library