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| Item Type: | Other |
|---|---|
| Title: | Dataset for: Modeling chemotherapy induced neurotoxicity with human induced pluripotent stem cell (iPSC)-derived sensory neurons |
| Creators Name: | Schinke, C., Fernandez Vallone, V., Ivanov, A., Peng, Y., Körtvelyessy, P., Nolte, L., Huehnchen, P., Beule, D., Stachelscheid, H., Boehmerle, W. and Endres, M. |
| Abstract: | Chemotherapy-induced peripheral neuropathy (CIPN) is a frequent and potentially irreversible adverse event of cytotoxic chemotherapy. We evaluate whether sensory neurons derived from induced pluripotent stem cells (iPSC-DSN) can serve as human disease model system for chemotherapy induced neurotoxicity. Sensory neurons differentiated from two established induced pluripotent stem cell lines were used (s.c. BIHi005-A https://hpscreg.eu/cell-line/BIHi005-A and BIHi004-B https://hpscreg.eu/cell-line/BIHi004-B, Berlin Institute of Health Stem Cell Core Facility). Cell viability and cytotoxicity assays were performed, comparing susceptibility to four neurotoxic and two non-neurotoxic drugs. RNA sequencing analyses in paclitaxel vs. vehicle (DMSO)-treated sensory neurons were performed. Treatment of iPSC-DSN for 24 h with the neurotoxic drugs paclitaxel, bortezomib, vincristine and cisplatin led to a dose dependent decline of cell viability in clinically relevant IC(50) ranges, which was not the case for the non-neurotoxic compounds doxorubicin and 5-fluorouracil. RNA sequencing analyses at 24 h, i.e. before paclitaxel-induced cell death occurred, revealed the differential expression of genes of neuronal injury, cellular stress response, and sterol pathways in response to 1 µM paclitaxel. Neuroprotective effects of lithium chloride co-incubation, which were previously shown in rodent dorsal root ganglia, could be replicated in human iPSC-DSN. Cell lines from the two different donors BIHi005-A and BIHi004-B showed different responses to the neurotoxic treatment in cell viability and cytotoxicity assays. |
| Keywords: | Induced Pluripotent Stem Cell Derived Sensory Neurons (iPSC-DSN), Chemotherapy Induced Neuropathy, 3R, Replacement, Transcriptome, Lithium |
| Source: | Data in Brief |
| ISSN: | 2352-3409 |
| Publisher: | Elsevier |
| Volume: | 38 |
| Page Range: | 107320 |
| Date: | October 2021 |
| Official Publication: | https://doi.org/10.1016/j.dib.2021.107320 |
| PubMed: | View item in PubMed |
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