Human brain organoids assemble functionally integrated bilateral optic vesicles

Tools

Item Type:	Article
Title:	Human brain organoids assemble functionally integrated bilateral optic vesicles
Creators Name:	Gabriel, E., Albanna, W., Pasquini, Gi., Ramani, A., Josipovic, N., Mariappan, A., Schinzel, F., Karch, C.M., Bao, G., Gottardo, M., Suren, A.A., Hescheler, J., Nagel-Wolfrum, K., Persico, V., Rizzoli, S.O., Altmüller, J., Riparbelli, M.G., Callaini, G., Goureau, O., Papantonis, A., Busskamp, V., Schneider, T. and Gopalakrishnan, J.
Abstract:	During embryogenesis, optic vesicles develop from the diencephalon via a multistep process of organogenesis. Using induced pluripotent stem cell (iPSC)-derived human brain organoids, we attempted to simplify the complexities and demonstrate formation of forebrain-associated bilateral optic vesicles, cellular diversity, and functionality. Around day 30, brain organoids attempt to assemble optic vesicles, which develop progressively as visible structures within 60 days. These optic vesicle-containing brain organoids (OVB-organoids) constitute a developing optic vesicle's cellular components, including primitive corneal epithelial and lens-like cells, retinal pigment epithelia, retinal progenitor cells, axon-like projections, and electrically active neuronal networks. OVB-organoids also display synapsin-1, CTIP-positive myelinated cortical neurons, and microglia. Interestingly, various light intensities could trigger photosensitive activity of OVB-organoids, and light sensitivities could be reset after transient photobleaching. Thus, brain organoids have the intrinsic ability to self-organize forebrain-associated primitive sensory structures in a topographically restricted manner and can allow interorgan interaction studies within a single organoid.
Keywords:	Brain Organoids, Optic Vesicles, Forebrain Organoids, iPSCs, Primordial Eye Fields, OVB-Organoids, FOXG1, Primary Cilium, Retinal Pigment Epithelium
Source:	Cell Stem Cell
ISSN:	1934-5909
Publisher:	Cell Press
Volume:	28
Number:	10
Page Range:	1740-1757.e8
Date:	October 2021
Official Publication:	https://doi.org/10.1016/j.stem.2021.07.010
PubMed:	View item in PubMed

Repository Staff Only: item control page