Apoptotic cleavage of DNA in human lymphocyte chromatin shows high sequence specificity

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Item Type:	Article
Title:	Apoptotic cleavage of DNA in human lymphocyte chromatin shows high sequence specificity
Creators Name:	Bettecken, T., Frenkel, Z.M., Altmüller, J., Nürnberg, P. and Trifonov, E.N.
Abstract:	Apoptotic digestion of human lymphocyte chromatin results in the appearance of large amounts of nucleosome size DNA fragments. Sequencing of these fragments and analysis of the distribution of bases around the apoptotic nucleases' cutting sites revealed a rather strong consensus sequence, not observed earlier. The consensus TAAAgTAcTTTA is characterized by complementary symmetry, resembling prokaryotic restriction sites. This consensus also possesses three TA dinucleotide steps, separated by five bases (corresponding to a half-period of the DNA double helix), suggesting strong bending of the DNA at the cut sites which is perhaps required for cutting.
Keywords:	Apoptosis, Base Sequence, Chromatin, DNA, DNA Restriction Enzymes, Lymphocytes, Molecular Sequence Data, Nucleosomes / Metabolism
Source:	Journal of Biomolecular Structure & Dynamics
ISSN:	1538-0254
Publisher:	Taylor & Francis
Volume:	30
Number:	2
Page Range:	211-6
Date:	12 June 2012
Official Publication:	https://doi.org/10.1080/07391102.2012.677772
PubMed:	View item in PubMed

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