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| Item Type: | Article |
|---|---|
| Title: | PRISMA and BioID disclose a motifs-based interactome of the intrinsically disordered transcription factor C/EBPα |
| Creators Name: | Ramberger, E., Sapozhnikova, V., Kowenz-Leutz, E., Zimmermann, K., Nicot, N., Nazarov, P.V., Perez-Hernandez, D., Reimer, U., Mertins, P., Dittmar, G. and Leutz, A. |
| Abstract: | C/EBPα represents a paradigm intrinsically disordered transcription factor containing short linear motifs and post-translational modifications (PTM). Unraveling C/EBPα protein interaction networks is a prerequisite for understanding the multi-modal functions of C/EBPα in hematopoiesis and leukemia. Here, we combined arrayed peptide matrix screening (PRISMA) with BioID to generate an in vivo validated and isoform specific interaction map of C/EBPα. The myeloid C/EBPα interactome comprises promiscuous and PTM-regulated interactions with protein machineries involved in gene expression, epigenetics, genome organization, DNA replication, RNA processing, and nuclear transport. C/EBPα interaction hotspots coincide with homologous conserved regions of the C/EBP family that also score as molecular recognition features. PTMs alter the interaction spectrum of C/EBP-motifs to configure a multi-valent transcription factor hub that interacts with multiple co-regulatory components, including BAF/SWI-SNF or Mediator complexes. Combining PRISMA and BioID is a powerful strategy to systematically explore the PTM-regulated interactomes of intrinsically disordered transcription factors. |
| Source: | iScience |
| ISSN: | 2589-0042 |
| Publisher: | Cell Press |
| Volume: | 24 |
| Number: | 6 |
| Page Range: | 102686 |
| Date: | 25 June 2021 |
| Official Publication: | https://doi.org/10.1016/j.isci.2021.102686 |
| PubMed: | View item in PubMed |
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