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| Item Type: | Article |
|---|---|
| Title: | AT1 and AT2 receptor knockout changed osteonectin and bone density in mice in periodontal inflammation experimental model |
| Creators Name: | Lima, M.L.S., Medeiros, C.A.C.X., Guerra, G.C.B., Santos, R., Bader, M., Pirih, F.Q., Araújo Júnior, R.F., Chan, A.B., Cruz, L.J., Brito, G.A.C., Leitão, R.F.C., Silveira, EJ.D., Garcia, V.B., Martins, A.A. and Araújo, A.A. |
| Abstract: | BACKGROUND: The aim of this study was to evaluate the role of AT1 and AT2 receptors in a periodontal inflammation experimental model. METHODS: Periodontal inflammation was induced by LPS/Porphyromonas gingivalis. Maxillae, femur, and vertebra were scanned using Micro-CT. Maxillae were analyzed histopathologically, immunohistochemically, and by RT-PCR. RESULTS: The vertebra showed decreased BMD in AT1 H compared with WT H (p < 0.05). The femur showed increased Tb.Sp for AT1 H and AT2 H, p < 0.01 and p < 0.05, respectively. The Tb.N was decreased in the vertebra (WT H-AT1 H: p < 0.05; WT H-AT2 H: p < 0.05) and in the femur (WT H-AT1 H: p < 0.01; WT H-AT2 H: p < 0.05). AT1 PD increased linear bone loss (p < 0.05) and decreased osteoblast cells (p < 0.05). RANKL immunostaining was intense for AT1 PD and WT PD (p < 0.001). OPG was intense in the WT H, WT PD, and AT2 PD when compared to AT1 PD (p < 0.001). AT1 PD showed weak immunostaining for osteocalcin compared with WT H, WT PD, and AT2 PD (p < 0.001). AT1 H showed significantly stronger immunostaining for osteonectin in fibroblasts compared to AT2 H (p < 0.01). CONCLUSION: AT1 receptor knockout changed bone density, the quality and number of bone trabeculae, decreased the number of osteoblast cells, and increased osteonectin in fibroblasts. |
| Keywords: | Bone, Micro-Computed Tomography, Periodontitis, Inflammation, Osteonectin, Animals, Mice |
| Source: | International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Publisher: | MDPI |
| Volume: | 22 |
| Number: | 10 |
| Page Range: | 5217 |
| Date: | 14 May 2021 |
| Official Publication: | https://doi.org/10.3390/ijms22105217 |
| PubMed: | View item in PubMed |
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