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| Item Type: | Article |
|---|---|
| Title: | The C. elegans pseudogene sspt-16 (F55A3.7) is required to safeguard germ cells against reprogramming |
| Creators Name: | Ofenbauer, A., Kraus, C.M. and Tursun, B. |
| Abstract: | We recently identified FAcilitates Chromatin Transcription (FACT) as a reprogramming barrier of transcription factor (TF) mediated conversion of germ cells into neurons in C. elegans. FACT is a conserved heterodimer consisting of SPT16 and SSRP1 in mammals. Duplication events during evolution in C. elegans generated two SSRP1 homologs named HMG-3 and HMG-4, while SPT-16 is the only homolog of SPT16. Yet, the pseudogene F55A3.7 has nearly complete nucleotide sequence homology to the spt-16 gene. However, F55A3.7 lacks some spt-16 exons and DNA pieces so we named it sspt-16 (short spt-16). Surprisingly, the deletion mutant ok1829, which affects only the sspt-16 pseudogene, shows similar germ cell reprogramming effects as described previously for FACT-depleted animals. We examined whether lack of sspt-16 affects other genes or chromatin accessibility, which may explain the permissiveness for germ cell reprogramming. |
| Keywords: | Animals, Caenorhabditis elegans |
| Source: | microPublication Biology |
| ISSN: | 2578-9430 |
| Publisher: | Caltech Library |
| Volume: | 2021 |
| Date: | 18 May 2021 |
| Official Publication: | https://doi.org/10.17912/micropub.biology.000392 |
| PubMed: | View item in PubMed |
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