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| Item Type: | Article |
|---|---|
| Title: | Spatio-temporal bone remodeling after hematopoietic stem cell transplantation |
| Creators Name: | Schwarz, C.S., Bucher, C.H., Schlundt, C., Mertlitz, S., Riesner, K., Kalupa, M., Verlaat, L., Schmidt-Bleek, O., Sass, R.A., Schmidt-Bleek, K., Duda, G.N., Penack, O. and Na, I.K. |
| Abstract: | The interaction of hematopoietic cells and the bone microenvironment to maintain bone homeostasis is increasingly appreciated. We hypothesized that the transfer of allogeneic T lymphocytes has extensive effects on bone biology and investigated trabecular and cortical bone structures, the osteoblast reconstitution, and the bone vasculature in experimental hematopoietic stem cell transplantations (HSCT). Allogeneic or syngeneic hematopoietic stem cells (HSC) and allogeneic T lymphocytes were isolated and transferred in a murine model. After 20, 40, and 60 days, bone structures were visualized using microCT and histology. Immune cells were monitored using flow cytometry and bone vessels, bone cells and immune cells were fluorescently stained and visualized. Remodeling of the bone substance, the bone vasculature and bone cell subsets were found to occur as early as day +20 after allogeneic HSCT (including allogeneic T lymphocytes) but not after syngeneic HSCT. We discovered that allogeneic HSCT (including allogeneic T lymphocytes) results in a transient increase of trabecular bone number and bone vessel density. This was paralleled by a cortical thinning as well as disruptive osteoblast lining and loss of B lymphocytes. In summary, our data demonstrate that the adoptive transfer of allogeneic HSCs and allogeneic T lymphocytes can induce profound structural and spatial changes of bone tissue homeostasis as well as bone marrow cell composition, underlining the importance of the adaptive immune system for maintaining a balanced bone biology. |
| Keywords: | Hematopoietic Stem Cell Transplantation, T Lymphocytes, Bone Remodeling, Animals, Mice |
| Source: | International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Publisher: | MDPI |
| Volume: | 22 |
| Number: | 1 |
| Page Range: | 267 |
| Date: | 29 December 2020 |
| Official Publication: | https://doi.org/10.3390/ijms22010267 |
| PubMed: | View item in PubMed |
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