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| Item Type: | Article |
|---|---|
| Title: | Photocaged Hoechst enables subnuclear visualization and cell selective staining of DNA in vivo |
| Creators Name: | Lämmle, C.A., Varady, A., Müller, T.G., Sturtzel, C., Riepl, M., Mathes, B., Eichhorst, J., Sporbert, A., Lehmann, M., Kräusslich, H.G., Distel, M. and Broichhagen, J. |
| Abstract: | Selective targeting of DNA by means of fluorescent labelling has become a mainstay in the life sciences. While genetic engineering serves as a powerful technique and allows for the visualization of nucleic acid by using DNA-targeting fluorescent fusion proteins in a cell-type and subcellular specific manner, it relies on the introduction of foreign genes. On the other hand, DNA-binding small fluorescent molecules can be used without genetic engineering but they are not spatially restricted. Here, we report a photocaged version of the DNA dye Hoechst33342 (pcHoechst), which can be uncaged using UV to blue light for the selective staining of chromosomal DNA in subnuclear regions of live cells. Expanding its application to a vertebrate model organism, we demonstrate uncaging in epithelial cells and short-term cell tracking in vivo in zebrafish. We envision pcHoechst as a valuable tool for targeting and interrogating DNA with precise spatiotemporal resolution in living cells and wild-type organisms. |
| Keywords: | DNA, Hoechst, Labelling, Microscopy, Animals, Zebrafish |
| Source: | ChemBioChem |
| ISSN: | 1439-4227 |
| Publisher: | Wiley |
| Volume: | 22 |
| Number: | 3 |
| Page Range: | 548-556 |
| Date: | 2 February 2021 |
| Official Publication: | https://doi.org/10.1002/cbic.202000465 |
| PubMed: | View item in PubMed |
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