Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy

[thumbnail of Original Article]
Preview
PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
7MB
[thumbnail of Supplementary Material] Other (Supplementary Material)
172MB

Item Type:Article
Title:Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy
Creators Name:Prahst, C., Ashrafzadeh, P., Mead, T., Figueiredo, A., Chang, K., Richardson, D., Venkaraman, L., Richards, M., Russo, A.M., Harrington, K., Ouarné, M., Pena, A., Chen, J.X., Claesson-Welsh, L., Cho, K.S., Franco, C.A. and Bentley, K.
Abstract:As the general population ages, more people are affected by eye diseases, such as retinopathies. It is therefore critical to improve imaging of eye disease mouse models. Here, we demonstrate that 1) rapid, quantitative 3D and 4D (time lapse) imaging of cellular and subcellular processes in the mouse eye is feasible, with and without tissue clearing, using light-sheet fluorescent microscopy (LSFM); 2) flat-mounting retinas for confocal microscopy significantly distorts tissue morphology, confirmed by quantitative correlative LSFM-Confocal imaging of vessels; 3) LSFM readily reveals new features of even well-studied eye disease mouse models, such as the oxygen-induced retinopathy (OIR) model, including a previously unappreciated 'knotted' morphology to pathological vascular tufts, abnormal cell motility and altered filopodia dynamics when live-imaged. We conclude that quantitative 3D/4D LSFM imaging and analysis has the potential to advance our understanding of the eye, in particular pathological, neurovascular, degenerative processes.
Keywords:Confocal Microscopy, Eye Diseases, Fluorescence Microscopy, Retina, Retinal Vessels, Three-Dimensional Imaging, Animals, Mice
Source:eLife
ISSN:2050-084X
Publisher:eLife Sciences Publications
Volume:9
Page Range:e49779
Date:19 February 2020
Official Publication:https://doi.org/10.7554/eLife.49779
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library