![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
Preview |
PDF (Original Article)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
2MB |
![[thumbnail of Supplementary Material]](https://edoc.mdc-berlin.de/style/images/fileicons/other.png) |
Other (Supplementary Material)
6MB |
| Item Type: | Article |
|---|---|
| Title: | The chloroplast RNA binding protein CP31A has a preference for mRNAs encoding the subunits of the chloroplast NAD(P)H dehydrogenase complex and is required for their accumulation |
| Creators: |
Lenzen, B.  ORCID: https://orcid.org/0000-0002-7881-9513, Rühle, T., Lehniger, M.K., Okuzaki, A. ORCID: https://orcid.org/0000-0003-1094-5748, Labs, M. ORCID: https://orcid.org/0000-0003-2104-2763, Muino, J.M. ORCID: https://orcid.org/0000-0002-6403-7262, Ohler, U. ORCID: https://orcid.org/0000-0002-0881-3116, Leister, D. and Schmitz-Linneweber, C. ORCID: https://orcid.org/0000-0002-6125-4253
|
| Abstract: | Chloroplast RNA processing requires a large number of nuclear-encoded RNA binding proteins (RBPs) that are imported post-translationally into the organelle. Most of these RBPs are highly specific for one or few target RNAs. By contrast, members of the chloroplast ribonucleoprotein family (cpRNPs) have a wider RNA target range. We here present a quantitative analysis of RNA targets of the cpRNP CP31A using digestion-optimized RNA co-immunoprecipitation with deep sequencing (DO-RIP-seq). This identifies the mRNAs coding for subunits of the chloroplast NAD(P)H dehydrogenase (NDH) complex as main targets for CP31A. We demonstrate using whole-genome gene expression analysis and targeted RNA gel blot hybridization that the ndh mRNAs are all down-regulated in cp31a mutants. This diminishes the activity of the NDH complex. Our findings demonstrate how a chloroplast RNA binding protein can combine functionally related RNAs into one post-transcriptional operon. |
| Keywords: | Chloroplast, RNA Processing, RNA Binding, NDH Complex, RRM, Organelle, Arabidopsis thaliana |
| Source: | International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Publisher: | MDPI |
| Volume: | 21 |
| Number: | 16 |
| Page Range: | 5633 |
| Date: | 6 August 2020 |
| Official Publication: | https://doi.org/10.3390/ijms21165633 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
