Chemodetection and destruction of host urea allows Helicobacter pylori to locate the epithelium

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Item Type:	Article
Title:	Chemodetection and destruction of host urea allows Helicobacter pylori to locate the epithelium
Creators Name:	Huang, J.Y., Sweeney Goers, E., Sigal, M., Zhang, H.C., Remington, S.J., Cantrell, M.A., Kuo, C.J., Guillemin, K. and Amieva, M.R.
Abstract:	The gastric pathogen Helicobacter pylori interacts intimately with the gastric mucosa to avoid the microbicidal acid in the stomach lumen. The cues H. pylori senses to locate and colonize the gastric epithelium have not been well defined. We show that metabolites emanating from human gastric organoids rapidly attract H. pylori. This response is largely controlled by the bacterial chemoreceptor TlpB, and the main attractant emanating from epithelia is urea. Our previous structural analyses show that TlpB binds urea with high affinity. Here we demonstrate that this tight binding controls highly sensitive responses, allowing detection of urea concentrations as low as 50 nM. Attraction to urea requires that H. pylori urease simultaneously destroys the signal. We propose that H. pylori has evolved a sensitive urea chemodetection and destruction system that allows the bacterium to dynamically and locally modify the host environment to locate the epithelium.
Keywords:	Animal Disease Models, Bacterial Proteins, Chemotaxis, Epithelium, Gastric Mucosa, Helicobacter Infections, Helicobacter pylori, Inbred C57BL Mice, Organ Culture Techniques, Urea, Urease, Animals, Mice
Source:	Cell Host & Microbe
ISSN:	1931-3128
Publisher:	Elsevier / Cell Press
Volume:	18
Number:	2
Page Range:	147-156
Date:	12 August 2015
Official Publication:	https://doi.org/10.1016/j.chom.2015.07.002
External Fulltext:	View full text on PubMed Central
PubMed:	View item in PubMed

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