![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
Preview |
PDF (Original Article)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
3MB |
![[thumbnail of Supplemental Information]](https://edoc.mdc-berlin.de/style/images/fileicons/other.png) |
Other (Supplemental Information)
20MB |
| Item Type: | Article |
|---|---|
| Title: | Network Rewiring of Homologous Recombination Enzymes during Mitotic Proliferation and Meiosis. |
| Creators Name: | Wild, P., Susperregui, A., Piazza, I., Dörig, C., Oke, A., Arter, M., Yamaguchi, M., Hilditch, A.T., Vuina, K., Chan, K.C., Gromova, T., Haber, J.E., Fung, J.C., Picotti, P. and Matos, J. |
| Abstract: | Homologous recombination (HR) is essential for high-fidelity DNA repair during mitotic proliferation and meiosis. Yet, context-specific modifications must tailor the recombination machinery to avoid (mitosis) or enforce (meiosis) the formation of reciprocal exchanges-crossovers-between recombining chromosomes. To obtain molecular insight into how crossover control is achieved, we affinity purified 7 DNA-processing enzymes that channel HR intermediates into crossovers or noncrossovers from vegetative cells or cells undergoing meiosis. Using mass spectrometry, we provide a global characterization of their composition and reveal mitosis- and meiosis-specific modules in the interaction networks. Functional analyses of meiosis-specific interactors of MutLγ-Exo1 identified Rtk1, Caf120, and Chd1 as regulators of crossing-over. Chd1, which transiently associates with Exo1 at the prophase-to-metaphase I transition, enables the formation of MutLγ-dependent crossovers through its conserved ability to bind and displace nucleosomes. Thus, rewiring of the HR network, coupled to chromatin remodeling, promotes context-specific control of the recombination outcome. |
| Keywords: | DNA Repair, Crossing-Over, Mlh1-Mlh3-Exo1, Sgs1(BLM)-Top3-Rmi1, Mus81-Mms4(EME1), Yen1(GEN1), Srs2(RTEL1), Slx1-Slx4(BTDB12), Mph1(FANCM), Holliday Junction |
| Source: | Molecular Cell |
| ISSN: | 1097-2765 |
| Publisher: | Cell Press |
| Volume: | 75 |
| Number: | 4 |
| Page Range: | 859-874 |
| Date: | 22 August 2019 |
| Official Publication: | https://doi.org/10.1016/j.molcel.2019.06.022 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
