Association of condensin with chromosomes depends on DNA binding by its HEAT-repeat subunits

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Item Type:	Article
Title:	Association of condensin with chromosomes depends on DNA binding by its HEAT-repeat subunits
Creators Name:	Piazza, I., Rutkowska, A., Ori, A., Walczak, M., Metz, J., Pelechano, V., Beck, M. and Haering, C.H.
Abstract:	Condensin complexes have central roles in the three-dimensional organization of chromosomes during cell divisions, but how they interact with chromatin to promote chromosome segregation is largely unknown. Previous work has suggested that condensin, in addition to encircling chromatin fibers topologically within the ring-shaped structure formed by its SMC and kleisin subunits, contacts DNA directly. Here we describe the discovery of a binding domain for double-stranded DNA formed by the two HEAT-repeat subunits of the Saccharomyces cerevisiae condensin complex. From detailed mapping data of the interfaces between the HEAT-repeat and kleisin subunits, we generated condensin complexes that lack one of the HEAT-repeat subunits and consequently fail to associate with chromosomes in yeast and human cells. The finding that DNA binding by condensin's HEAT-repeat subunits stimulates the SMC ATPase activity suggests a multistep mechanism for the loading of condensin onto chromosomes.
Keywords:	Adenosine Triphosphatases, Binding Sites, Chromosomes, DNA, DNA-Binding Proteins, Molecular Models, Molecular Sequence Data, Multiprotein Complexes, Protein Sequence Analysis, Protein Subunits, Saccharomyces Cerevisiae, Sequence Alignment
Source:	Nature Structural & Molecular Biology
ISSN:	1545-9993
Publisher:	Nature Publishing Group
Volume:	21
Number:	6
Page Range:	560-568
Date:	June 2014
Official Publication:	https://doi.org/10.1038/nsmb.2831
PubMed:	View item in PubMed

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