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Biochemical and functional characterization of the murine ros protooncogene

Item Type:Article
Title:Biochemical and functional characterization of the murine ros protooncogene
Creators Name:Riethmacher, D., Langholz, O., Goedecke, S., Sachs, M. and Birchmeier, C.
Abstract:The ros gene was originally found because it can, when mutated, induce malignant transformation. The protooncogene encodes an orphan receptor tyrosine kinase. We report here the isolation and characterization of the mouse c-ros cDNA and, in addition, the biochemical characterization of the receptor. Both, the endogenous c-ros protein from embryonal tissues and the recombinant protein are glycosylated molecules with an apparent molecular weight of 260,000. Pulse-chase analysis in Sf9 cells demonstrates that the c-ros protein is synthesized as a single chain, uncleaved molecule. Since the specific ligand of c-ros is not known, a hybrid receptor (trk/c-ros) which transmits c-ros-specific signals in response to nerve growth factor (NGF) was used to study the biological activities. In NIH3T3 cells, this trk/c-ros hybrid induces growth, a fusiform cell shape, and loss of contact inhibition of growth. However, the active hybrid receptor cannot replace IL-3 as survival factor in 32D myeloid cells. Compared to other receptors, the active c-ros tyrosine kinase domain displays thus overlapping, but not identical signalling specificities.
Keywords:3T3 Cells, Amino Acid Sequence, Baculoviridae, Cultured Cells, Molecular Sequence Data, Proto-Oncogene Proteins, Receptor Protein-Tyrosine Kinases, Signal Transduction, Spodoptera, Animals, Mice
Publisher:Nature Publishing Group
Page Range:3617-3626
Date:December 1994
PubMed:View item in PubMed

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