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| Item Type: | Article |
|---|---|
| Title: | Unique properties of PTEN-L contribute to neuroprotection in response to ischemic-like stress |
| Creators Name: | Jochner, M.C.E., An, J., Lättig-Tünnemann, G., Kirchner, M., Dagane, A., Dittmar, G., Dirnagl, U., Eickholt, B.J. and Harms, C. |
| Abstract: | Phosphatase and tensin homolog (PTEN) signalling might influence neuronal survival after brain ischemia. However, the influence of the less studied longer variant termed PTEN-L (or PTENα) has not been studied to date. Therefore, we examined the translational variant PTEN-L in the context of neuronal survival. We identified PTEN-L by proteomics in murine neuronal cultures and brain lysates and established a novel model to analyse PTEN or PTEN-L variants independently in vitro while avoiding overexpression. We found that PTEN-L, unlike PTEN, localises predominantly in the cytosol and translocates to the nucleus 10-20 minutes after glutamate stress. Genomic ablation of PTEN and PTEN-L increased neuronal susceptibility to oxygen-glucose deprivation. This effect was rescued by expression of either PTEN-L indicating that both PTEN isoforms might contribute to a neuroprotective response. However, in direct comparison, PTEN-L replaced neurons were protected against ischemic-like stress compared to neurons expressing PTEN. Neurons expressing strictly nuclear PTEN-L NLS showed increased vulnerability, indicating that nuclear PTEN-L alone is not sufficient in protecting against stress. We identified mutually exclusive binding partners of PTEN-L or PTEN in cytosolic or nuclear fractions, which were regulated after ischemic-like stress. GRB2-associated-binding protein 2, which is known to interact with phosphoinositol-3-kinase, was enriched specifically with PTEN-L in the cytosol in proximity to the plasma membrane and their interaction was lost after glutamate exposure. The present study revealed that PTEN and PTEN-L have distinct functions in response to stress and might be involved in different mechanisms of neuroprotection. |
| Keywords: | Animal Disease Models, Brain, Brain Ischemia, Cell Nucleus, GRB2 Adaptor Protein, Gene Expression Regulation, Glucose, Neurons, Neuroprotection, Oxygen, Protein Isoforms, PTEN Phosphohydrolase, Proteomics, Signal Transducing Adaptor Proteins, Signal Transduction, Stroke, Animals, Mice |
| Source: | Scientific Reports |
| ISSN: | 2045-2322 |
| Publisher: | Nature Publishing Group |
| Volume: | 9 |
| Number: | 1 |
| Page Range: | 3183 |
| Date: | 28 February 2019 |
| Official Publication: | https://doi.org/10.1038/s41598-019-39438-1 |
| PubMed: | View item in PubMed |
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