![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
| Item Type: | Article |
|---|---|
| Title: | Spatial transcriptomics of C. elegans males and hermaphrodites identifies sex-specific differences in gene expression patterns |
| Creators Name: | Ebbing, A., Vértesy, Á., Betist, M.C., Spanjaard, B., Junker, J.P., Berezikov, E., van Oudenaarden, A. and Korswagen, H.C. |
| Abstract: | To advance our understanding of the genetic programs that drive cell and tissue specialization, it is necessary to obtain a comprehensive overview of gene expression patterns. Here, we have used spatial transcriptomics to generate high-resolution, anteroposterior gene expression maps of C. elegans males and hermaphrodites. To explore these maps, we have developed computational methods for discovering region- and tissue-specific genes. We have found extensive sex-specific gene expression differences in the germline and sperm and discovered genes that are specifically expressed in the male reproductive tract. These include a group of uncharacterized genes that encode small secreted proteins that are required for male fertility. We conclude that spatial gene expression maps provide a powerful resource for identifying tissue-specific gene functions in C. elegans. Importantly, we found that expression maps from different animals can be precisely aligned, enabling transcriptome-wide comparisons of gene expression patterns. |
| Keywords: | C. elegans, Spatial Transcriptomics, mRNA Sequencing, CEL-Seq, Germline, Sperm, Male Fertility, Animals, Caenorhabditis elegans |
| Source: | Developmental Cell |
| ISSN: | 1534-5807 |
| Publisher: | Cell Press |
| Volume: | 47 |
| Number: | 6 |
| Page Range: | 801-813 |
| Date: | 17 December 2018 |
| Official Publication: | https://doi.org/10.1016/j.devcel.2018.10.016 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
