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Dendrite-specific amplification of weak synaptic input during network activity in vivo

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Item Type:Article
Title:Dendrite-specific amplification of weak synaptic input during network activity in vivo
Creators Name:Ferrarese, L., Jouhanneau, J.S., Remme, M.W.H., Kremkow, J., Katona, G., Rózsa, B., Schreiber, S. and Poulet, J.F.A.
Abstract:Excitatory synaptic input reaches the soma of a cortical excitatory pyramidal neuron via anatomically segregated apical and basal dendrites. In vivo, dendritic inputs are integrated during depolarized network activity, but how network activity affects apical and basal inputs is not understood. Using subcellular two-photon stimulation of Channelrhodopsin2-expressing layer 2/3 pyramidal neurons in somatosensory cortex, nucleus-specific thalamic optogenetic stimulation, and paired recordings, we show that slow, depolarized network activity amplifies small-amplitude synaptic inputs targeted to basal dendrites but reduces the amplitude of all inputs from apical dendrites and the cell soma. Intracellular pharmacology and mathematical modeling suggests that the amplification of weak basal inputs is mediated by postsynaptic voltage-gated channels. Thus, network activity dynamically reconfigures the relative somatic contribution of apical and basal inputs and could act to enhance the detectability of weak synaptic inputs.
Keywords:Action Potentials, Cultured Cells, Dendrites, Excitatory Postsynaptic Potentials, Inbred C57BL Mice, Neurological Models, Pyramidal Cells, Somatosensory Cortex, Thalamus, Animals, Mice
Source:Cell Reports
Publisher:Cell Press / Elsevier
Page Range:3455-3465
Date:25 September 2018
Official Publication:https://doi.org/10.1016/j.celrep.2018.08.088
PubMed:View item in PubMed

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