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| Item Type: | Article |
|---|---|
| Title: | Kinase-controlled phase transition of membraneless organelles in mitosis |
| Creators Name: | Rai, A.K., Chen, J.X., Selbach, M. and Pelkmans, L. |
| Abstract: | Liquid-liquid phase separation has been shown to underlie the formation and disassembly of membraneless organelles in cells, but the cellular mechanisms that control this phenomenon are poorly understood. A prominent example of regulated and reversible segregation of liquid phases may occur during mitosis, when membraneless organelles disappear upon nuclear-envelope breakdown and reappear as mitosis is completed. Here we show that the dual-specificity kinase DYRK3 acts as a central dissolvase of several types of membraneless organelle during mitosis. DYRK3 kinase activity is essential to prevent the unmixing of the mitotic cytoplasm into aberrant liquid-like hybrid organelles and the over-nucleation of spindle bodies. Our work supports a mechanism in which the dilution of phase-separating proteins during nuclear-envelope breakdown and the DYRK3-dependent degree of their solubility combine to allow cells to dissolve and condense several membraneless organelles during mitosis. |
| Keywords: | Anaphase-Promoting Complex-Cyclosome, Cytoplasm, Cytoplasmic Granules, Hek293 Cells, Hela Cells, Mitosis, Nuclear Envelope, Organelles, Physiological, Stress, Poly(A)-Binding Protein I, Protein Binding, Protein Transport, Protein-Serine-Threonine Kinases, Protein-Tyrosine Kinases, Solubility, Spindle Apparatus |
| Source: | Nature |
| ISSN: | 0028-0836 |
| Publisher: | Nature Publishing Group |
| Volume: | 559 |
| Number: | 7713 |
| Page Range: | 211-216 |
| Date: | July 2018 |
| Official Publication: | https://doi.org/10.1038/s41586-018-0279-8 |
| PubMed: | View item in PubMed |
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