Kinase-controlled phase transition of membraneless organelles in mitosis

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Item Type:	Article
Title:	Kinase-controlled phase transition of membraneless organelles in mitosis
Creators Name:	Rai, A.K., Chen, J.X., Selbach, M. and Pelkmans, L.
Abstract:	Liquid-liquid phase separation has been shown to underlie the formation and disassembly of membraneless organelles in cells, but the cellular mechanisms that control this phenomenon are poorly understood. A prominent example of regulated and reversible segregation of liquid phases may occur during mitosis, when membraneless organelles disappear upon nuclear-envelope breakdown and reappear as mitosis is completed. Here we show that the dual-specificity kinase DYRK3 acts as a central dissolvase of several types of membraneless organelle during mitosis. DYRK3 kinase activity is essential to prevent the unmixing of the mitotic cytoplasm into aberrant liquid-like hybrid organelles and the over-nucleation of spindle bodies. Our work supports a mechanism in which the dilution of phase-separating proteins during nuclear-envelope breakdown and the DYRK3-dependent degree of their solubility combine to allow cells to dissolve and condense several membraneless organelles during mitosis.
Keywords:	Anaphase-Promoting Complex-Cyclosome, Cytoplasm, Cytoplasmic Granules, Hek293 Cells, Hela Cells, Mitosis, Nuclear Envelope, Organelles, Physiological, Stress, Poly(A)-Binding Protein I, Protein Binding, Protein Transport, Protein-Serine-Threonine Kinases, Protein-Tyrosine Kinases, Solubility, Spindle Apparatus
Source:	Nature
ISSN:	0028-0836
Publisher:	Nature Publishing Group
Volume:	559
Number:	7713
Page Range:	211-216
Date:	July 2018
Official Publication:	https://doi.org/10.1038/s41586-018-0279-8
PubMed:	View item in PubMed

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