Human induced pluripotent stem cell production and expansion from blood using a non-integrating viral reprogramming vector

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Item Type:	Article
Title:	Human induced pluripotent stem cell production and expansion from blood using a non-integrating viral reprogramming vector
Creators Name:	Sharma, A., Mücke, M. and Seidman, C.E.
Abstract:	We describe a method to transform blood lymphocytes into human-induced pluripotent stem cells by delivering four transcription factors with a non-integrative virus. Using human peripheral blood mononuclear cells (PBMCs) as the source cell type for hiPSC reprogramming is advantageous since blood samples are rapidly and safely obtained from nearly-all subjects. Reprogramming factors needed to make hiPSCs are introduced by infecting the PBMCs with non-integrating Sendai virus vectors. Reprogrammed cells can subsequently be quickly expanded for downstream use. In this unit, we present current protocols for the isolation of PBMCs from a small sample of human blood and subsequent viral reprogramming and expansion of PBMCs into hiPSCs.
Keywords:	PBMC, Sendai Virus, Cell Fate, iPSCs, Pluripotent Stem Cells, Reprogramming
Source:	Current Protocols in Molecular Biology
ISSN:	1934-3639
Publisher:	Wiley-Blackwell
Volume:	122
Number:	1
Page Range:	e58
Date:	April 2018
Official Publication:	https://doi.org/10.1002/cpmb.58
External Fulltext:	View full text on PubMed Central
PubMed:	View item in PubMed

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