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G protein subunit G alpha16 expression is restricted to progenitor B-cells during human B-cell differentiation

Item Type:Article
Title:G protein subunit G alpha16 expression is restricted to progenitor B-cells during human B-cell differentiation
Creators Name:Mapara, M.Y., Bommert, K., Bargou, R.C., Leng, C., Beck, C., Ludwig, W.D., Gierschik, P. and Doerken, B.
Abstract:Recently G alpha 16, a new guanosine triphosphate (GTP) binding protein alpha subunit has been described to be specifically expressed in human hematopoietic cells. Expression of G alpha 16 was observed in human cell lines of myelomonocytic and T-lymphocytic origin, but not in human B-cell lines Raji and IM9. We studied the expression of G alpha 16 in human B cells corresponding to different stages of B-cell differentiation by means of reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting. The human Burkitt's lymphoma cell lines Raji, Ramos, BJAB, the lymphoblastoid cell line SKW6.4, and the plasmocytoma cell line U266 were devoid of G alpha 16. In contrast, G alpha 16 was detected in the human progenitor B cell lines Reh and Nalm-6. Using the mu+, k- cell line BLIN-1 (pre-B cell phenotype) and its derived subclone 1E8 (surface mu+, k+; B-cell phenotype) G alpha 16 expression was found to disappear on transition from pre-B to B-cell differentiation stage. The analysis of a broad panel of human neoplastic B lymphocytes ranging from progenitor B-acute lymphatic leukemia (pre-pre-B-ALL), common acute leukemias (cALL), pre-B-ALL, mature B-ALL to low grade B-cell lymphoma (chronic lymphocytic leukemia of B-cell type, leukemic centrocytic non-Hodgkins lymphoma [NHL], hairy cell leukemia) showed that G alpha 16 expression is limited to progenitor and pre-B-ALL cells. Therefore, we conclude that within B-cell differentiation, G alpha 16 is expressed solely during early B cell ontogeny and downregulated during differentiation. Thus, G alpha 16 might be an important regulator involved in signaling processes in progenitor B cells.
Keywords:B-Lymphocytes, Base Sequence, Burkitt Lymphoma, Cell Differentiation, GTP-Binding Proteins, Leukemic Gene Expression Regulation, Neoplastic Gene Expression Regulation, Immunophenotyping, B-Cell Leukemia, Hairy Cell Leukemia, B-Cell Chronic Lymphocytic Leukemia, Acute Promyelocytic Leukemia, Non-Hodgkin Lymphoma, Molecular Sequence Data, Neoplasm Proteins, Neoplastic Stem Cells, Plasmacytoma, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma, Protein Conformation, B-Cell Antigen Receptors, Signal Transduction, Cultured Tumor Cells
Publisher:American Society of Hematology
Page Range:1836-1842
Date:1 April 1995
Official Publication:http://bloodjournal.hematologylibrary.org/cgi/content/abstract/85/7/1836
PubMed:View item in PubMed

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