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| Item Type: | Article |
|---|---|
| Title: | Cell type atlas and lineage tree of a whole complex animal by single-cell transcriptomics |
| Creators: |
Plass, M.  ORCID: https://orcid.org/0000-0002-9891-2723, Solana, J. ORCID: https://orcid.org/0000-0002-6770-3929, Wolf, F.A., Ayoub, S. ORCID: https://orcid.org/0000-0001-5043-8922, Misios, A., Glažar, P. ORCID: https://orcid.org/0000-0003-0842-0088, Obermayer, B. ORCID: https://orcid.org/0000-0002-9116-630X, Theis, F.J., Kocks, C. ORCID: https://orcid.org/0000-0003-1749-3334 and Rajewsky, N. ORCID: https://orcid.org/0000-0002-4785-4332
|
| Abstract: | Flatworms of the species are immortal-adult animals contain a large pool of pluripotent stem cells that continuously differentiate to all adult cell types. Therefore, single-cell transcriptome profiling of adult animals should reveal mature and progenitor cells. By combining perturbation experiments, gene expression analysis, a computational method that predicts future cell states from the transcriptional changes, and a lineage reconstruction method, we placed all major cell types onto a single lineage tree that connects all cells to a single stem cell compartment. We characterize gene expression changes during differentiation and discover cell types important for regeneration. Our results demonstrate the importance of single-cell transcriptome analysis for mapping and reconstructing fundamental processes of developmental and regenerative biology at high resolution. |
| Keywords: | Atlases As Topic, Cell Differentiation, Cell Lineage, Cells, Gene Expression Profiling, Planarians, Pluripotent Stem Cells, Regeneration, Single-Cell Analysis, Transcriptome, Animals |
| Source: | Science |
| ISSN: | 0036-8075 |
| Publisher: | American Association for the Advancement of Science |
| Volume: | 360 |
| Number: | 6391 |
| Page Range: | eaaq1723 |
| Date: | 25 May 2018 |
| Official Publication: | https://doi.org/10.1126/science.aaq1723 |
| PubMed: | View item in PubMed |
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