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Cell type atlas and lineage tree of a whole complex animal by single-cell transcriptomics

Item Type:Article
Title:Cell type atlas and lineage tree of a whole complex animal by single-cell transcriptomics
Creators Name:Plass, M., Solana, J., Wolf, F.A., Ayoub, S., Misios, A., Glažar, P., Obermayer, B., Theis, F.J., Kocks, C. and Rajewsky, N.
Abstract:Flatworms of the species are immortal-adult animals contain a large pool of pluripotent stem cells that continuously differentiate to all adult cell types. Therefore, single-cell transcriptome profiling of adult animals should reveal mature and progenitor cells. By combining perturbation experiments, gene expression analysis, a computational method that predicts future cell states from the transcriptional changes, and a lineage reconstruction method, we placed all major cell types onto a single lineage tree that connects all cells to a single stem cell compartment. We characterize gene expression changes during differentiation and discover cell types important for regeneration. Our results demonstrate the importance of single-cell transcriptome analysis for mapping and reconstructing fundamental processes of developmental and regenerative biology at high resolution.
Keywords:Atlases As Topic, Cell Differentiation, Cell Lineage, Cells, Gene Expression Profiling, Planarians, Pluripotent Stem Cells, Regeneration, Single-Cell Analysis, Transcriptome, Animals
Publisher:American Association for the Advancement of Science
Page Range:eaaq1723
Date:25 May 2018
Official Publication:https://doi.org/10.1126/science.aaq1723
PubMed:View item in PubMed

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