Athymic mice reveal a requirement for T-cell-microglia interactions in establishing a microenvironment supportive of Nf1 low-grade glioma growth

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Item Type:	Article
Title:	Athymic mice reveal a requirement for T-cell-microglia interactions in establishing a microenvironment supportive of Nf1 low-grade glioma growth
Creators Name:	Pan, Y., Xiong, M., Chen, R., Ma, Y., Corman, C., Maricos, M., Kindler, U., Semtner, M., Chen, Y.H., Dahiya, S. and Gutmann, D.H.
Abstract:	Pediatric low-grade gliomas (LGGs) frequently do not engraft in immunocompromised mice, limiting their use as an experimental platform. In contrast, murine Neurofibromatosis-1 (Nf1) optic LGG stem cells (o-GSCs) form glioma-like lesions in wild-type, but not athymic, mice following transplantation. Here, we show that the inability of athymic mice to support o-GSC engraftment results from impaired microglia/macrophage function, including reduced expression of Ccr2 and Ccl5, both of which are required for o-GSC engraftment and Nf1 optic glioma growth. Impaired Ccr2 and Ccl5 expression in athymic microglia/macrophages was restored by T-cell exposure, establishing T-cell-microglia/macrophage interactions as critical stromal determinants that support NF1 LGG growth.
Keywords:	Tumor Microenvironment, Stroma, Monocyte, Chemokines, Animals, Mice
Source:	Genes & Development
ISSN:	0890-9369
Publisher:	Cold Spring Harbor Laboratory Press
Volume:	32
Number:	7-8
Page Range:	491-496
Date:	9 April 2018
Official Publication:	https://doi.org/10.1101/gad.310797.117
External Fulltext:	View full text on PubMed Central
PubMed:	View item in PubMed

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