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Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb

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Item Type:Article
Title:Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb
Creators Name:Wolff, C., Tinevez, J.Y., Pietzsch, T., Stamataki, E., Harich, B., Guignard, L., Preibisch, S., Shorte, S., Keller, P.J., Tomancak, P. and Pavlopoulos, A.
Abstract:During development, coordinated cell behaviors orchestrate tissue and organ morphogenesis. Detailed descriptions of cell lineages and behaviors provide a powerful framework to elucidate the mechanisms of morphogenesis. To study the cellular basis of limb development, we imaged transgenic fluorescently-labeled embryos from the crustacean Parhyale hawaiensis with multi-view light-sheet microscopy at high spatiotemporal resolution over several days of embryogenesis. The cell lineage of outgrowing thoracic limbs was reconstructed at single-cell resolution with new software called Massive Multi-view Tracker (MaMuT). In silico clonal analyses suggested that the early limb primordium becomes subdivided into anterior-posterior and dorsal-ventral compartments whose boundaries intersect at the distal tip of the growing limb. Limb-bud formation is associated with spatial modulation of cell proliferation, while limb elongation is also driven by preferential orientation of cell divisions along the proximal-distal growth axis. Cellular reconstructions were predictive of the expression patterns of limb development genes including the BMP morphogen Decapentaplegic.
Keywords:Amphipoda, Cell Lineage, Computational Biology, Computer-Assisted Image Processing, Extremities, Fluorescence, Reporter Genes, Morphogenesis, Optical Imaging, Software, Spatio-Temporal Analysis, Staining and Labeling, Animals
Publisher:eLife Sciences Publications
Page Range:e34410
Date:29 March 2018
Official Publication:https://doi.org/10.7554/eLife.34410
PubMed:View item in PubMed

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