Ultrastructural localization of adhesion molecules in the healthy and inflamed choroid plexus of the mouse

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Item Type:	Article
Title:	Ultrastructural localization of adhesion molecules in the healthy and inflamed choroid plexus of the mouse
Creators Name:	Wolburg, K., Gerhardt, H., Schulz, M., Wolburg, H. and Engelhardt, B.
Abstract:	The functional expression of vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1) and MAdCAM-1 in the choroid plexus is indicative of a role of this structure in the communication of the immune system with the central nervous system (CNS). In order to gain further insight into the possible functions of adhesion molecules expressed in the choroid plexus, we investigated the exact ultrastructural localization of VCAM-1, ICAM-1 and MAdCAM-1 on semithin and ultrathin cryosections of the choroid plexus of healthy mice and of mice suffering from experimental autoimmune encephalomyelitis (EAE). In the healthy choroid plexus VCAM-1 and ICAM-1, but not MAdCAM-1, could be detected on the apical surface of the choroid plexus epithelial cells. During EAE, immunoreactivity for VCAM-1 and ICAM-1 was dramatically increased. Additionally, apical expression of MAdCAM-1 was observed on individual choroid plexus epithelial cells during EAE. At the same time, VCAM-1, ICAM-1 or MAdCAM-1 were never present on the endothelial cells of the fenestrated capillaries within the choroid plexus. The polar expression of VCAM-1, ICAM-1 and MAdCAM-1 on the apical surface of choroid plexus epithelial cells, which form the blood-cerebrospinal fluid barrier, implies a previously unappreciated function of this barrier in the immunosurveillance of the CNS.
Keywords:	Choroid Plexus, ICAM-1, VCAM-1, MAdCAM-1, Experimental Autoimmune, Encephalomyelitis, Mouse (SJL/N), Animals, Mice
Source:	Cell and Tissue Research
ISSN:	0302-766X
Publisher:	Springer
Volume:	296
Number:	2
Page Range:	259-269
Date:	April 1999
Official Publication:	https://doi.org/10.1007/s004410051287
PubMed:	View item in PubMed

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