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| Item Type: | Article |
|---|---|
| Title: | Endothelial cell O-glycan deficiency causes blood/lymphatic misconnections and consequent fatty liver disease in mice |
| Creators: |
Fu, J., Gerhardt, H.  ORCID: https://orcid.org/0000-0002-3030-0384, McDaniel, J.M., Xia, B., Liu, X., Ivanciu, L., Ny, A., Hermans, K., Silasi-Mansat, R. ORCID: https://orcid.org/0000-0001-9590-6160, McGee, S., Nye, E., Ju, T., Ramirez, M.I. ORCID: https://orcid.org/0000-0001-6294-4419, Carmeliet, P., Cummings, R.D., Lupu, F. ORCID: https://orcid.org/0000-0003-1249-9278 and Xia, L.
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| Abstract: | Mucin-type O-glycans (O-glycans) are highly expressed in vascular ECs. However, it is not known whether they are important for vascular development. To investigate the roles of EC O-glycans, we generated mice lacking T-synthase, a glycosyltransferase encoded by the gene C1galt1 that is critical for the biosynthesis of core 1-derived O-glycans, in ECs and hematopoietic cells (termed here EHC T-syn(-/-) mice). EHC T-syn(-/-) mice exhibited embryonic and neonatal lethality associated with disorganized and blood-filled lymphatic vessels. Bone marrow transplantation and EC C1galt1 transgene rescue demonstrated that lymphangiogenesis specifically requires EC O-glycans, and intestinal lymphatic microvessels in EHC T-syn(-/-) mice expressed a mosaic of blood and lymphatic EC markers. The level of O-glycoprotein podoplanin was significantly reduced in EHC T-syn(-/-) lymphatics, and podoplanin-deficient mice developed blood-filled lymphatics resembling EHC T-syn(-/-) defects. In addition, postnatal inactivation of C1galt1 caused blood/lymphatic vessel misconnections that were similar to the vascular defects in the EHC T-syn(-/-) mice. One consequence of eliminating T-synthase in ECs and hematopoietic cells was that the EHC T-syn(-/-) pups developed fatty liver disease, because of direct chylomicron deposition via misconnected portal vein and intestinal lymphatic systems. Our studies therefore demonstrate that EC O-glycans control the separation of blood and lymphatic vessels during embryonic and postnatal development, in part by regulating podoplanin expression. |
| Keywords: | Cultured Cells, Endothelial Cells, Fatty Liver, Galactosyltransferases, Lymphatic Vessels, Microvessels, Transgenes, Transgenic Mice, Animals, Mice |
| Source: | Journal of Clinical Investigation |
| ISSN: | 0021-9738 |
| Publisher: | American Society for Clinical Investigation |
| Volume: | 118 |
| Number: | 11 |
| Page Range: | 3725-3737 |
| Date: | November 2008 |
| Official Publication: | https://doi.org/10.1172/JCI36077 |
| PubMed: | View item in PubMed |
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