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Autologous iPSC-based vaccines elicit anti-tumor responses in vivo

Item Type:Article
Title:Autologous iPSC-based vaccines elicit anti-tumor responses in vivo
Creators Name:Kooreman, N.G., Kim, Y., de Almeida, P.E., Termglinchan, V., Diecke, S., Shao, N.Y., Wei, T.T., Yi, H., Dey, De., Nelakanti, R., Brouwer, T.P., Paik, D.T., Sagiv-Barfi, I., Han, A., Quax, P.H.A., Hamming, J.F., Levy, R., Davis, M.M. and Wu, J.C.
Abstract:Cancer cells and embryonic tissues share a number of cellular and molecular properties, suggesting that induced pluripotent stem cells (iPSCs) may be harnessed to elicit anti-tumor responses in cancer vaccines. RNA sequencing revealed that human and murine iPSCs express tumor-associated antigens, and we show here a proof of principle for using irradiated iPSCs in autologous anti-tumor vaccines. In a prophylactic setting, iPSC vaccines prevent tumor growth in syngeneic murine breast cancer, mesothelioma, and melanoma models. As an adjuvant, the iPSC vaccine inhibited melanoma recurrence at the resection site and reduced metastatic tumor load, which was associated with fewer Th17 cells and increased CD11bGR1myeloid cells. Adoptive transfer of T cells isolated from vaccine-treated tumor-bearing mice inhibited tumor growth in unvaccinated recipients, indicating that the iPSC vaccine promotes an antigen-specific anti-tumor T cell response. Our data suggest an easy, generalizable strategy for multiple types of cancer that could prove highly valuable in clinical immunotherapy.
Keywords:Pluripotent Stem Cells, Immunotherapy, Prophylactic Vaccination, Adjuvant Therapy, Shared Epitopes, Breast Cancer, Melanoma, Mesothelioma, Metastases, Immune Profiling, Animals, Mice
Source:Cell Stem Cell
Publisher:Cell Press
Page Range:501-513
Date:5 April 2018
Official Publication:https://doi.org/10.1016/j.stem.2018.01.016
External Fulltext:View full text on PubMed Central
PubMed:View item in PubMed

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