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A gain-of-function mutation in the CLCN2 chloride channel gene causes primary aldosteronism

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Item Type:Article
Title:A gain-of-function mutation in the CLCN2 chloride channel gene causes primary aldosteronism
Creators Name:Fernandes-Rosa, F.L., Daniil, G., Orozco, I.J., Göppner, C., El Zein, R., Jain, V., Boulkroun, S., Jeunemaitre, X., Amar, L., Lefebvre, H., Schwarzmayr, T., Strom, T.M., Jentsch, T.J. and Zennaro, M.C.
Abstract:Primary aldosteronism is the most common and curable form of secondary arterial hypertension. We performed whole-exome sequencing in patients with early-onset primary aldosteronism and identified a de novo heterozygous c.71G>A/p.Gly24Asp mutation in the CLCN2 gene, encoding the voltage-gated ClC-2 chloride channel 1 , in a patient diagnosed at 9 years of age. Patch-clamp analysis of glomerulosa cells of mouse adrenal gland slices showed hyperpolarization-activated Cl- currents that were abolished in Clcn2-/- mice. The p.Gly24Asp variant, located in a well-conserved 'inactivation domain'2,3, abolished the voltage- and time-dependent gating of ClC-2 and strongly increased Cl- conductance at resting potentials. Expression of ClC-2Asp24 in adrenocortical cells increased expression of aldosterone synthase and aldosterone production. Our data indicate that CLCN2 mutations cause primary aldosteronism. They highlight the important role of chloride in aldosterone biosynthesis and identify ClC-2 as the foremost chloride conductor of resting glomerulosa cells.
Keywords:Chloride Channels, Cohort Studies, DNA Mutational Analysis, Gain of Function Mutation, Germ-Line Mutation, Hyperaldosteronism, Knockout Mice, Pedigree, Whole Exome Sequencing, Zona Glomerulosa, Animals, Mice
Source:Nature Genetics
Publisher:Nature Publishing Group
Page Range:355-361
Date:March 2018
Additional Information:Copyright © 2018 Nature America Inc., part of Springer Nature. All rights reserved.
Official Publication:https://doi.org/10.1038/s41588-018-0053-8
PubMed:View item in PubMed

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