Helmholtz Gemeinschaft


Complete suppression of Htt fibrilization and disaggregation of Htt fibrils by a trimeric chaperone complex

Item Type:Article
Title:Complete suppression of Htt fibrilization and disaggregation of Htt fibrils by a trimeric chaperone complex
Creators Name:Scior, A., Buntru, A., Arnsburg, K., Ast, A., Iburg, M., Juenemann, K., Pigazzini, M.L., Mlody, B., Puchkov, D., Priller, J., Wanker, E.E., Prigione, A. and Kirstein, J.
Abstract:Huntington's disease (HD) is a neurodegenerative disorder caused by an expanded CAG trinucleotide repeat in the huntingtin gene (HTT). Molecular chaperones have been implicated in suppressing or delaying the aggregation of mutant Htt. Using in vitro and in vivo assays, we have identified a trimeric chaperone complex (Hsc70, Hsp110, and J-protein) that completely suppresses fibrilization of HttExon1Q(48) The composition of this chaperone complex is variable as recruitment of different chaperone family members forms distinct functional complexes. The trimeric chaperone complex is also able to resolubilize Htt fibrils. We confirmed the biological significance of these findings in HD patient-derived neural cells and on an organismal level in Caenorhabditis elegans Among the proteins in this chaperone complex, the J-protein is the concentration-limiting factor. The single overexpression of DNAJB1 in HEK293T cells is sufficient to profoundly reduce HttExon1Q(97) aggregation and represents a target of future therapeutic avenues for HD.
Keywords:Disaggregation, HttpolyQ, Molecular Chaperones, NPCs, Suppression, Animals, Caenorhabditis elegans
Source:EMBO Journal
Publisher:EMBO Press / Wiley
Page Range:282-299
Date:17 January 2018
Additional Information:Erratum in: EMBO J 40:e109413.
Official Publication:https://doi.org/10.15252/embj.201797212
External Fulltext:View full text on PubMed Central
PubMed:View item in PubMed

Repository Staff Only: item control page

Open Access
MDC Library