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Methods for lineage tracing on the organism-wide level

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Item Type:Review
Title:Methods for lineage tracing on the organism-wide level
Creators Name:Spanjaard, B. and Junker, J.P.
Abstract:Determining the lineage origin of cell types is a major goal in developmental biology. Furthermore, lineage tracing is a powerful approach for understanding the origin of developmental defects as well as the origin of diseases such as cancer. There is now a variety of complementary approaches for identifying lineage relationships, ranging from direct observation of cell divisions by light microscopy to genetic labeling of cells using inducible recombinases and fluorescent reporters. A recent development, and the main topic of this review article, is the use of high-throughput sequencing data for lineage analysis. This emerging approach holds the promise of increased multiplexing capacity, allowing lineage analysis of large cell numbers up to the organism-wide level combined with simultaneous transcription profiling by single cell RNA sequencing.
Keywords:Cell Lineage
Source:Current Opinion in Cell Biology
Publisher:Elsevier / Current Biology
Page Range:16-21
Date:December 2017
Additional Information:Copyright © 2017 Elsevier
Official Publication:https://doi.org/10.1016/j.ceb.2017.11.004
PubMed:View item in PubMed

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