Transcriptome-wide identification of protein binding sites on RNA by PAR-CLIP (photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation)

Tools

Item Type:	Book Section
Title:	Transcriptome-wide identification of protein binding sites on RNA by PAR-CLIP (photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation)
Creators Name:	Hoell, J.I., Hafner, M., Landthaler, M., Ascano, M., Farazi, T.A., Wardle, G., Nusbaum, J., Cekan, P., Khorshid, M., Burger, L., Zavolan, M. and Tuschl, T.
Keywords:	Immunoprecipitation, Crosslinking, 4-Thiouridine (4SU), 6-Thioguanosine (6SG), Preadenylated Adapter, RNA Size Markers, cDNA Library Preparation, Radiolabeling of RNA Size Markers, RNA Binding Protein (RBP), UV-Croslinking of 4SU-Labeled Cells, Magnetic Beads for Immunoprecipitation, RNase T1, SDS-PAGE, Electroelution of RNA-RBP Complexes, Proteinase K, Adapter Ligation, Reverse Transcription, PCR, Illumina Sequencing of cDNAs, Reversed Phase HPLC (RP-HPLC), Determination of 4SU Incorporation Levels
Source:	Handbook of RNA Biochemistry: Second, Completely Revised and Enlarged Edition
Title of Book:	Handbook of RNA Biochemistry: Second, Completely Revised and Enlarged Edition
ISBN:	9783527327645
Publisher:	Wiley-VCH
Volume:	2
Page Range:	877-898
Date:	2014
Official Publication:	https://doi.org/10.1002/9783527647064.ch39

Repository Staff Only: item control page