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| Item Type: | Article |
|---|---|
| Title: | Modulation of the endogenous omega-3 fatty acid and oxylipin profile in vivo - a comparison of the fat-1 transgenic mouse with C57BL/6 wildtype mice on an omega-3 fatty acid enriched diet |
| Creators Name: | Ostermann, A.I., Waindok, P., Schmidt, M.J., Chiu, C.Y., Smyl, C., Rohwer, N., Weylandt, K.H. and Schebb, N.H. |
| Abstract: | Dietary intervention and genetic fat-1 mice are two models for the investigation of effects associated with omega-3 polyunsaturated fatty acids (n3-PUFA). In order to assess their power to modulate the fatty acid and oxylipin pattern, we thoroughly compared fat-1 and wild-type C57BL/6 mice on a sunflower oil diet with wild-type mice on the same diet enriched with 1% EPA and 1% DHA for 0, 7, 14, 30 and 45 days. Feeding led after 14-30 days to a high steady state of n3-PUFA in all tissues at the expense of n6-PUFAs. Levels of n3-PUFA achieved by feeding were higher compared to fat-1 mice, particularly for EPA (max. 1.7% in whole blood of fat-1 vs. 7.8% following feeding). Changes in PUFAs were reflected in most oxylipins in plasma, brain and colon: Compared to wild-type mice on a standard diet, arachidonic acid metabolites were overall decreased while EPA and DHA oxylipins increased with feeding more than in fat-1 mice. In plasma of n3-PUFA fed animals, EPA and DHA metabolites from the lipoxygenase and cytochrome P450 pathways dominated over ARA derived counterparts.Fat-1 mice show n3-PUFA level which can be reached by dietary interventions, supporting the applicability of this model in n3-PUFA research. However, for specific questions, e.g. the role of EPA derived mediators or concentration dependent effects of (individual) PUFA, feeding studies are necessary. |
| Keywords: | Body Weight, Diet, Fatty Acid Desaturases, Fatty Acids, Omega-3, Oxylipins, Animals, Mice |
| Source: | PLoS ONE |
| ISSN: | 1932-6203 |
| Publisher: | Public Library of Science |
| Volume: | 12 |
| Number: | 9 |
| Page Range: | e0184470 |
| Date: | 8 September 2017 |
| Official Publication: | https://doi.org/10.1371/journal.pone.0184470 |
| PubMed: | View item in PubMed |
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